Figure 2. TSC1 and S6 kinase deletion does not cause DNA damage‐associated foci.

• A, B
  Tsc1 ^fl/fl and Tsc1 ^fl/fl S6K1 ^−/− S6K2 ^−/− primary MEFs were transduced with GFP or GFP‐Cre adenovirus. Twelve days post‐infection (passage P7) cells were fixed and analysed by immunofluorescence using the indicated antibodies. The formation of γH2AX or 53BP1 foci per cell was analysed in three independent experiments. Control cells treated with etoposide (6 μg/ml) overnight were used as a positive control (n = 3 cultures). Scale bars: 5 μm.