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HEK293T cells expressing Orai1‐CFP and STIM1‐YFP were transfected with either control siRNA or NCLX siRNA and effect on Orai1/STIM1 corrected FRET after store depletion with 2.5 μM ionomycin (Iono) were compared. Bar scale: warmer colors indicate high FRET. The scale bar represents 10 μm.
