Figure EV2. The R151A, I155A mutations of syntaxin‐1 have no effect on ternary SNARE complex formation with syntaxin‐1, SNAP‐25, and synaptobrevin‐2 (i.e. in the absence of Munc18‐1 and the MUN domain).

• A, B
  Ternary SNARE complex formation as monitored by ensemble FRET efficiency between fluorescent dye‐labeled SNAP‐25 and the cytoplasmic domain of synaptobrevin‐2 (A) and quantification of the results (B). Neg. ctrl. (negative control): The experiment was performed in the absence of syntaxin‐1. Shown are means ± SD for n = 3 technical replicates.