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. 2017 Jan 30;36(6):816–829. doi: 10.15252/embj.201695775

Figure EV5. Effect of the MUN domain containing the NFAA (N1128A, F1131A) mutations on conformational changes of syntaxin‐1 bound to Munc18‐1.

Figure EV5

smFRET efficiency histograms and FRET efficiency values (bar chart) corresponding to the peak positions of the Gaussian functions fit to the smFRET efficiency histograms for the specified syntaxin‐1 label pairs and conditions, using the same procedure as in Fig 4. The bar charts show mean values ± SD for the two subsets of an equal partition of the data that are comprised of the observed FRET efficiency values for all molecules in each different condition (see Appendix Tables S2 and S3). Shown are smFRET efficiency histograms for the cytoplasmic domain of syntaxin‐1 bound to Munc18‐1, upon the addition of the NFAA mutant of the MUN domain, upon the simultaneous addition of SNAP‐25, the cytoplasmic domain of synaptobrevin‐2, and the NFAA mutant of the MUN domain. S25, SNAP‐25; SB, synaptobrevin‐2; M18, Munc18‐1.