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WT MEFs were treated with HMW poly(I:C), LMW poly(I:C), or thapsigargin (th) for 4 h, prior to a chase of 0.5, 1, 2, and 3 h with fresh medium. When indicated, cycloheximide (CHX) +/− MG132 were added to the medium during the chase. GADD34, PKR, p‐eIF2α, and total eIF2α were detected by immunoblot with specific antibodies.
