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. 2004 Dec 15;114(12):1782–1789. doi: 10.1172/JCI18046

Figure 6.

Figure 6

siRNApyk2 decreases Pyk2 protein abundance in OKP cells. Typical blots. (A) Cells were transfected with 1.33 μg/well siRNApyk2 or siRNAGL2 (GL2 siRNA) or transfection reagent only (No siRNA) for 24 hours (at which time they were confluent), rendered quiescent for 24 hours, and harvested. FAK protein abundance was measured for comparison. (B) Cells were transfected with 1.33 μg/well siRNApyk2 for 24 hours (at which time they were confluent) and rendered quiescent. The length of quiescence varied from 0 to 48 hours, so that cells were harvested 24, 48, or 72 hours after the beginning of transfection. (C) Cells were transfected with the indicated amount of siRNApyk2 for 24 hours (at which time they were confluent), rendered quiescent for 24 hours, and harvested.