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. 2004 Dec 15;114(12):1704–1713. doi: 10.1172/JCI20427

Figure 2.

Figure 2

Osteoclast function in caspase-3–deficient mice. (A) Representative images of titanium-induced resorption in the calvarial bones (white arrows) of WT, Casp3+/–, and Casp3–/– mice. There was a significant decrease in bone resorption in Casp3–/– mice compared with WT mice (WT, n = 12; Casp3+/–, n = 12; Casp3–/–, n = 8; #P < 0.05). (B) The number of induced TRAP+-MNCs. Spleen cells from WT mice were cocultured with preosteoblasts from WT, Casp3+/–, and Casp3–/– mice. The number of TRAP+-MNCs was significantly decreased in Casp3–/– and Casp3+/– preosteoblasts compared with WT (+/+) preosteoblasts following treatment with 0.5 nM or 2 nM 1,25(OH)2D3 (n = 6; *P < 0.001; #P < 0.05). (C) Expression of RANKL. RANKL was downregulated in Casp3–/– preosteoblasts compared with WT preosteoblasts by Western blot analysis. The expression of HSP90 is shown as a control for each protein loading.