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. 2004 Dec 15;114(12):1752–1761. doi: 10.1172/JCI21625

Figure 3.

Figure 3

Dysregulated expressions of adipose genes and increased expressions of NADPH oxidase subunits in WAT of obese KKAy mice. (A) The mRNA expressions of adiponectin, TNF-α, PAI-1, and PPARγ in WAT of C57BL/6 (white bars) and KKAy (black bars) mice. The mRNA amounts were quantified by real-time PCR. Values are normalized to the level of cyclophilin mRNA, and expressed as mean ± SEM (n = 6–8). (B) The mRNA expressions of NADPH oxidase subunits in various mouse tissues. The mRNA amounts were quantified by real-time PCR, using total RNA extracted from 8 tissues of C57BL/6 mice at 12 weeks of age. Values are normalized to the level of 18S ribosomal RNA. (C and D) The mRNA expressions of NADPH oxidase subunits and PU.1 in WAT (C), liver (D, left) and skeletal muscle (D, right) of C57BL/6 (white bars) and KKAy (black bars) mice at 7 and 13 weeks of age. Values are normalized to the level of cyclophilin mRNA, and expressed as mean ± SEM (n = 6–8). **P < 0.01 and ***P < 0.001 compared with C57BL/6 mice. BAT, brown adipose tissue.