Skip to main content
. 2004 Dec 15;114(12):1752–1761. doi: 10.1172/JCI21625

Figure 5.

Figure 5

Production of ROS in 3T3-L1 adipocytes. (A) ROS production during differentiation of 3T3-L1 cells into adipocytes. ROS production was measured by NBT reduction. Oil red O staining (top) and NBT treatment (middle) of the cells. Dark-blue formazan was dissolved and the absorbance was determined at 560 nm (bottom). (B) Effect of linoleate and inhibitors of ROS production in fully differentiated 3T3-L1 adipocytes. 3T3-L1 adipocytes were incubated with (FFA+) or without (FFA–) 200 μM linoleate for 24 hours. In the final hour of incubation, 10 mM NAC, 10 μM DPI, 200 μM apocynin, 100 μM oxypurinol, 100 μM rotenone, or 100 μM thenoyltrifluoroacetone (TTFA) was added, and ROS production was measured by NBT reduction. Values are expressed as mean ± SEM (n = 3). ***P < 0.001 compared with cells without linoleate or inhibitors. ###P < 0.001 compared with cells treated with 200 μM linoleate.