Figure 6.

Effects of ROS on gene expressions in 3T3-L1 adipocytes. (A and B) The mRNA expression levels of adiponectin, PAI-1, PPARγ, IL-6, and MCP-1 in 3T3-L1 adipocytes exposed to ROS, with or without antioxidant NAC. Fully differentiated 3T3-L1 adipocytes were exposed to ROS by incubation with H₂O₂. Values are normalized to the level of cyclophilin mRNA and expressed as mean ± SEM (n = 3). (C) The mRNA expression levels of NADPH oxidase subunits and PU.1 in 3T3-L1 adipocytes exposed to ROS. Values are normalized to the level of cyclophilin mRNA. (D) Effects of H₂O₂ and NAC on adiponectin secretion from 3T3-L1 adipocytes. Adiponectin levels in the media were determined by Western blotting (inset) and the values were quantified using a densitometer. (E) Effects of ROS and NAC on the transcriptional activity of adiponectin promoter in 3T3-L1 adipocytes. RLU, relative luciferase units. Values are expressed as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001 compared with control.