Figure 9.
Effect of LPA1 overexpression in MDA-BO2 cells on osteoclast activity in vivo. (A) Animals fed without or with doxycycline were inoculated intravenously with MDA-BO2 or transfectants (clone no. 3 and clone no. 79 cells). Representative histological examination of TRAP-stained proximal tibia section from metastatic animals 30 days after tumor cell inoculation. Lower panels show magnified areas (white squares) from upper panels. Bone is stained in dark blue and osteoclasts are stained in red (arrows). Scale bar: 200 μm. (B) The Oc.S/BS ratio was quantified. Results are the mean ± SD of 4–6 animals per group. *P < 0.001 doxycycline-free versus doxycycline-fed animals.