Figure 4.

Depletion of CD3⁺ cells from PBMCs reduces to background levels the IFN-γ response of the NK cells to fluA. CD3⁺ cells were depleted from the PBMCs of 8 donors (PBMC-CD3). PBMCs and CD3-deleted PBMCs were incubated with fluA for 17 hours, followed by intracellular staining for IFN-γ. Displayed in the bar graphs are frequencies of IFN-γ⁺ cells in the CD56^bright perforin^– (left) and CD56^dimperforin⁺ (right) NK cell subsets. Results of 3 donors (nos. 4–6) are presented in this figure, while those of other donors are presented in Figures 5 and 9 as part of other experiments.