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. 2004 Dec 15;114(12):1812–1819. doi: 10.1172/JCI22797

Figure 7.

Figure 7

IFN-γ and IL-2 production by NK cells and T cells in response to fluA. PBMCs from adult donors were incubated with fluA or SPG (negative control) for 12 hours, with brefeldin A added during the last 5 hours (A and B) or were incubated with fluA for 4–12 hours, with brefeldin A added for the last 4 hours (C). The cells were stained for CD56, fixed and permeabilized, and then stained intracellularly for CD3, IFN-γ, and IL-2. (A) Dot plots for a representative donor (no. 7) displaying cells gated on CD3–CD56+ NK cell population (left panels) or CD3+ T cell population (right panels). Numbers in the dot plots refer to the percentage of cytokine-producing cells in each quadrant. (B) Summary of the levels of IFN-γ and IL-2 production by T cells from 4 donors (nos. 7–10). IL-2 was not detected in NK cells from any of these 4 donors. (C) Kinetics of IL-2 production by T cells and IFN-γ production by NK cells (donor no. 20).