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. 2009 Sep 10;2(5):325–332. doi: 10.1111/j.1752-8062.2009.00141.x

Figure 6.

Figure 6

CaMK activity in human nonfailing versus failing LV. (A) The activity of CaMK was assessed in failing and nonfailing LVs. CaM kinase activity is approximately double that in the normal heart. CaMKII protein levels are unchanged in nonfailing versus FHHs. Protein levels in ten nonfailing and eight failing human LV total lysates were measured. Homogenized tissue was fractionated on a 10% SDS‐PAGE gel, transferred to nitrocellulose and immunoblotted with an antibody to CaMKII. (B) The upper band shows the presence of CaMKII at around 56kDa. On the same blot, the GAPDH signal shows equal loading in all lanes. (C) The accompanying graph shows that the ratio of CaMKII/ GAPDH integrated intensity values in not different in nonfailing and failing hearts.