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. 2009 Sep 10;2(5):325–332. doi: 10.1111/j.1752-8062.2009.00141.x

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© 2009 Wiley Periodicals, Inc.

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PKD activity as assessed by the detection of phosphorylatyed serine 910 signal is highly increased in the nuclear fraction in the failing versus nonfailing LVs. Nuclear and cytosolic fractions from eight nonfailing and eight failing LVs were loaded on an 8% SDS‐PAGE gel. (A) Detection of PKD pS910 was obtained with a rabbit antibody and the proteins were visualized with enhanced chemiluminescence. (B) The results normalized to their corresponding nonfailing compartment values were graphed.