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. 2009 Feb 18;2(1):41–49. doi: 10.1111/j.1752-8062.2008.00076.x

Figure 2.

Figure 2

Figure 2

Effect of resuscitation, IL‐6 treatment, and GQ‐ODN pretreatment on T/HS‐induced lung apoptosis. Rats were subjected to sham protocol (Sham, n= 4), unresuscitated T/HS (UHS, n= 3), T/HS treated with placebo at the beginning of resuscitation (SBR50, n= 4), T/HS treated with IL‐6 at the beginning of resuscitation (SBR50/IL‐6, n= 5), T/HS preceded by treatment with GQ oligodeoxynucleotide (GQ‐ODN) 24 hours prior to resuscitation with IL‐6 (SBR50/IL‐6/G, n= 5), or T/HS preceded by treatment with nonspecific ODN (NS‐ODN) 24 hours prior to resuscitation with IL‐6 (SBR50/I‐6/N, n= 5). The lungs were harvested 60 minutes after the start of resuscitation. (A) Nucleosome levels were measured in protein extracts of frozen sections of each lung. Data presented are mean ± SEM of nucleosome level corrected for total protein for each group. Bars marked with an asterisk (*) differ significantly within the pair (p < 0.05, one‐way ANOVA). (B) Sections of paraformaldehyde‐fixed lung were stained using the TUNEL assay. Representative photomicrographs of 1,000× fields of lung specimens from each experimental group are shown. Apoptotic nuclei are indicated by arrows. Type I alveolar epithelial cells (AEC) are indicated by the number symbol (#). Type II AECs are indicated by an asterisk (*). (C) TUNEL‐positive nuclei were counted. Data shown are the mean ± SEM percentage of TUNEL‐positive nuclei per 1,000× fields (20 fields counted). Bars marked with an asterisk (*) differ significantly within the pair (p < 0.05).