Figure 1.

VEGF regulation of GRK5 in HCAECs. (A) HCAECs starved for 20 hours were treated with VEGF (20 ng/ mL) and harvested at different time points as indicated. Whole‐cell lysates were subjected to SDS‐PAGE and analyzed by Western blotting with anti‐GRK5 antibody. The blot was stripped and reprobed with GAPDH as a loading control. (B) HCAECs were infected with GRK5 or GFP adenovirus for 20 hours as described in Methods and then treated with VEGF (20 ng/mL) for indicated times. Whole‐cell lysates were subjected to Western blotting for GRK5 and GAPDH, as described above. (C) HCAECs were starved for 20 hours and challenged with VEGF (20 ng/mL). Total RNA was extracted. Expression of GRK5 mRNA was quantified by real‐time PCR. Data are mean ± SD for mRNA encoding GRK5 relative to that of 18S rRNA in triplicate.