Figure 4.

Effect of GRK5 on VEGF‐induced signaling in HCAECs. (A and B) HCAECs were infected with GRK5 adenovirus or one of the control viruses, GFP or LacZ. After 20 hours of starvation, the cells were treated with VEGF (20 ng/mL) for the indicated times. Whole‐cell lysates were subjected to SDS‐PAGE and Western blot analysis using antiphospho‐Akt and antiphospho‐ERK1/2 antibody. Total Akt and total ERK were detected as internal controls. Phosphorylation of GSK‐3β was also detected using the antiphospho‐GSK‐3β antibody. GRK5 overexpression is confirmed.