Figure 7.
Spermidine has no general effect on turnover rates of proteolytic substrates. (A) Steady-state levels of proteolytic test substrate detected by anti-ha Western blotting (upper part). Anti-Cdc11 loading control (lower part). βgal variants were expressed as ubiquitin fusions (Ub-X-βgal). (B) Quantitation of data in (A). (C) Pulse-chase detection of Ub-P-βgal turnover rates. (D) Quantitation of data in (C).