Table 1.

Primer sets used for MSP

Primer set	Sense primer	Antisense primer	Size (bp)/AT	References
p15‐M	5’‐GCGTTCGTATTTTGCGGTT‐3’	5’‐CGTACAATAACCGAACGACCGA‐3’	148 bp/57°C	20
p15‐U	5’‐TGTGATGTGTTTGTATTTTGTGGTT‐3’	5’‐CCATACAATAACCAAACAACCAA‐3’	154 bp/57°C	20
p16‐M	5’‐TTATTAGAGGGTGGGGCGGATCGC‐3’	5’‐GACCCCGAACCGCGACCGTAA‐3’	150 bp/65°C	20
p16‐U	5’‐TTATTAGAGGGTGGGGTGGATTGT‐3’	5’‐CAACCCCAAACCACAACCATAA‐3’	151 bp/60°C	20
MGMT‐M	5’‐TTTCGACGTTCGTAGGTTTTCGC‐3’	5’‐GCACTCTTCCGAAAACGAAACG‐3’	81 bp/57°C	21
MGMT‐U	5’‐TTTGTGTTTGATGTTTGTAGGTTTTTGT‐3’	5’‐AACTCCACACTCTTCCAAAAACAAAAA‐3’	93 bp/57°C	21
DAPK‐M	5’‐GGATAGTCGGATCGAGTTAACGTC‐3’	5’‐CCCTCCCAAACGCCGA‐3’	98 bp/60°C	22
DAPK‐U	5’‐GGAGGATAGTTGGATTGAGTTAATGTT‐3’	5’‐CAAATCCCTCCCAAACACCAA‐3’	106 bp/60°C	22

M = primer set for methylated modified DNA sequence; U = primer set for unmethylated modified DNA sequence; AT = annealing temperature.