Figure 5.

Cell cycle-dependent association of Trrap and HATs with the Mad1 and Mad2 promoters. Trrap-containing and Trrap-deficient cells were synchronized at indicated cell cycle phases as in Figure 2B, and analyzed by ChIP assay using anti-Trrap, anti-Tip60 and anti-PCAF antibodies. Immunoprecipitated DNA was analyzed by PCR using primers recognizing the Mad2 promoter (A) and the Mad1 promoter (B). Mock immunoprecipitation without addition of antibodies (No Ab.) was also included as a control. Input corresponds to PCR reactions containing 1/3750 of the total amount of chromatin used in immunoprecipitation reactions.