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. 2015 May 22;8(4):298–304. doi: 10.1111/cts.12275

Figure 2.

Figure 2

Sodium channel blockers prilocaine and procainamide decrease DMPK protein (67 KDa isoform) in vitro. C2C12 cells were treated with sodium channel blockers for 4 hours and then harvested for Western blot analysis. Representative Western blot (A) and densitometric quantification of DMPK protein (B and C) relative to tubulin (the ratio of control group is set as 1) upon treatment with mexiletine (50 nM). Representative Western blot (D) and densitometric quantification of DMPK protein (E and F) relative to tubulin (the ratio of control group is set as 1) upon treatment with prilocaine (1 μM). Representative Western blot (G) and densitometric quantification of DMPK protein (H and I) relative to tubulin (the ratio of control group is set as 1) upon treatment with procainamide (1 μM). Mean ± SEM (bars) of three independent experiments are shown. All lanes were run on the same gel but were noncontiguous. *p < 0.05; **p < 0.01; ***p < 0.001, t‐test.