Fig. 1.

Microglial-derived MP are increased in the blood following TBI. Flow cytometry analysis of enriched MP in the blood from sham and TBI mice at 24 h post-injury. a Representation of gating strategy used to characterize MP using SSC-H and standard microbeads (300- to 1000-nm diameter). Standard microbeads (P1 gated population) were used as an internal control to determine the size of MP in the blood, and annexin V staining confirmed MP characteristics. At 24 h post-injury, total blood MP is increased in TBI mice compared with sham-injured mice. b Measurements of leukocyte-derived (CD18), macrophage-derived (F4/80), and microglial-derived (P2Y12/CD45) MP in the blood from sham and TBI mice at 24 h post-injury. Microglial-derived MP are significantly increased in TBI mice when compared with sham-injured mice (*p < 0.5 vs sham; Student’s t test; n = 6/group). Bars represent mean ± standard error of the mean (S.E.M.). Data represent results of three independent experiments