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. 2017 Mar 14;17:63. doi: 10.1186/s12870-017-1011-9

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Stability of the BP178 peptide accumulated in 3 years-old seeds. The endosperm tissue was manually dissected from mature seeds (0-days) and germinating rice seeds (3 and 7 days of germination). Protein extracts (35 μg) were separated by Tricine-SDS gels, transferred onto PVDF membrane, and incubated with the anti-BP178 antibody followed by anti-rabbit IgG (Fc) alkaline phosphatase conjugated secondary antibody. EV, rice plants with the empty pCAMBIA 1300 vector were used as a negative control, and 3.2 μg of synthetic BP178 were used as reference control. Arrows, indicate BP178 A-related bands in rice extracts. Numbers in the left side indicate the size of the molecular markers (kDa). Lower panels show Ponceau staining of protein samples