Table 1.
Sequences of primers used in real‐time PCR reactions
| Small RNA‐specific forward primers | Accession Number (Database) | |
|---|---|---|
| RNU48 | 5'‐TGATGATGACCCCAGGTAACTCT‐3' | NR_002745.1 (GenBank) |
| miR‐183 | 5'‐TATGGCACTGGTAGAATTCACT‐3' | MIMAT0000261 (miRBase) |
| miR‐96 | 5'‐TGGCACTAGCACATTTTTGCTAAA‐3' | MIMAT0000095 (miRBase) |
| miR‐182 | 5'‐TTTGGCAATGGTAGAACTCACA‐3' | MIMAT0000259 (miRBase) |
| miR‐143 | 5'‐TGAGATGAAGCACTGTAGCTCAAA ‐3' | MIMAT0000435 (miRBase) |
| miR‐145 | 5'‐CCAGTTTTCCCAGGAATCCCTAA‐3' | MIMAT0000437 (miRBase) |
| Universal reverse primer | 5'‐GCGAGCACAGAATTAATACGAC‐3' |