Figure 4.

IL-17/IL-17R induces autophagy via the JAK2/STAT3 signaling pathway. SMMC-7721 cells were treated with 20 µg/ml Oxa in the presence or absence of (A) anti-IL-17R (10 µg/ml) or (B) IL-17 (200 ng/ml) for 18 h. (C) The activation of JAK2 and STAT3 was detected by western blotting. (D) LY294002 (10 µg/ml) and AG490 (15 µg/ml) were added to the culture medium for 1 h prior to Oxa treatment. LC3 and Beclin-1 protein expression was determined by western blot analysis. GAPDH was used as an internal control. Oxa, oxaliplatin; IL-17, interleukin-17; IL-17R, IL-17 receptor; JAK2, janus kinase 2; STAT3, signal transducer and activator of transcription 3; LC3, microtubule-associated protein 1 light chain.