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. 2016 Dec 20;13(2):613–620. doi: 10.3892/ol.2016.5519

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Resveratrol induced apoptosis in renal carcinoma cells. (A) Cytotoxicity of resveratrol was determined by a lactate dehydrogenase assay. (B) Caspase 3/7 activity subsequent to resveratrol treatment was determined by a Caspase-Glo 3/7 assay. (C) Resveratrol-induced apoptosis in renal carcinoma cells with different concentrations (0, 50, 100 and 200 µM) for 24 h, which was analyzed by flow cytometry. (D) Cells were treated with resveratrol (0, 50, 100 and 200 µM) for 24 h, and the levels of caspase 9, Bax and Bcl-2 were detected by western blotting. The data showed that resveratrol regulated apoptotic proteins in renal carcinoma cells in a dose-dependent manner. **P<0.01, experimental vs. control groups. JS-K, O²-(2,4-dinitrophenyl)1-[(4-ethoxyxarbonyl) piperazin-1-yl]diazen-1-ium-1,2-diolate; FITC, fluorescein isothiocyanate; Bcl-2, B-cell lymphoma 2; Bax, bcl-2-like protein 4; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.