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. 2017 Mar 14;17:29. doi: 10.1186/s12896-017-0349-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — The viability assays of cassava mesophyll protoplasts using the optimized protocol, i.e. preparation of mesophyll protoplasts in an enzyme solution (1.6% cellulase + 0.8% macerozyme) incubated at 25 °C (45 rpm) for 16 h in darkness. a Bright field image of protoplasts; (b) Protoplasts stained with FDA; (c) Merged image of protoplasts (Scale bar = 100 μm).