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. 2016 Dec 6;13(2):587–592. doi: 10.3892/ol.2016.5461

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Copyright: © He et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Overexpression of DKK4 in 6 colorectal cancer cell lines. (A) Reverse transcription-quantitative polymerase chain reaction analysis of DKK4 mRNA relative expression and (B) western blot analysis of DKK4 protein levels in cell lines following treatment with 5-Fu, YN968D1, both or neither. GAPDH was used as the internal control. DKK4, dickkopf-related protein 4; OE, overexpressing; Ctrl, control; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. Data is presented as the mean ± standard error. **P<0.005.