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Effect of apigenin and cisplatin on caspase activation. (A) A549 or (B) H1299 cells were treated with 30 µM apigenin for 2 h, followed by 10 µM cisplatin for another 48 h. Casp-3, −8 and −9 expression and their cleavage or PARP degradation in the two cell lines was evaluated by western blotting. (C) Semi-quantification of (A). (D) Semi-quantification of (B). Casp, caspase; PARP, poly ADP ribose polymerase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.**P<0.01.
