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. 2016 Dec 16;13(2):800–804. doi: 10.3892/ol.2016.5510

Figure 4.

Figure 4.

Expression levels of matrix metalloproteinase. HLF cells (A) and PLC/PRF/5 cells (B) were cultured in 6-well plates, and added to 3 µM sorafenib (+), 1 µM 2DG (+), or a combination of both. Following 48 h in culture, RNA was isolated and subjected to RT-qPCR. Levels of matrix metalloproteinase expression were analyzed. *P<0.05 against sorafenib (−) and 2DG (−), **P<0.05 against sorafenib (−) and 2DG (−), sorafenib (−) and 2DG (+), and sorafenib (+) and 2DG (−), ***P<0.05 against sorafenib (−) and 2DG (−), and sorafenib (−) and 2DG (+), n=3. HLF, Human liver fibroblast cells; 2DG, 2-deoxyglucose; PLC/PRF/5 cells, human hepatoma carcinoma cell line; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.