FIG. 2.
Detection of proteolytic activity during infection of G. mellonella larvae with the Brecon/1 strain. Protease activity in the hemolymph of infected larvae and in larva homogenates was analyzed by SDS-PAGE-coupled zymography (A), by native PAGE-coupled zymography (B), and by chromogen substrate hydrolysis (Fua-LGPA-ase activity) in hemolymph samples (C). In panels A and B, the same samples were analyzed, and the lanes in each panel represent samples from different larvae. The type of sample (cell culture supernatant, hemolymph, or body homogenate) and the time that the sample was taken (in hours postinfection) are indicated above the panels. In panel C, the activities in 1.0-μl hemolymph samples are plotted as a function of infection time. For the method used to determine kobs, see Materials and Methods.