TABLE 3.
Enzyme treatmenta | Fua-Ala-Leu-Val-Tyr hydrolysis (Absorbance/min, 10−5) |
---|---|
None | 6.08 |
EDTA (1 mM) | 6.45 |
PMSF (1 mM) | 4.50 |
Cysteine (1 mM) | 0.76 |
DTT (1 mM) | 0.00 |
PMSF, phenylmethylsulfonyl fluoride; DTT, 1,4-dithiothreitol.
Enzyme treatmenta | Fua-Ala-Leu-Val-Tyr hydrolysis (Absorbance/min, 10−5) |
---|---|
None | 6.08 |
EDTA (1 mM) | 6.45 |
PMSF (1 mM) | 4.50 |
Cysteine (1 mM) | 0.76 |
DTT (1 mM) | 0.00 |
PMSF, phenylmethylsulfonyl fluoride; DTT, 1,4-dithiothreitol.