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Relation between normalized fluorescence intensity of fluorescein-labeled probes (13) and ΔG°overall. (a) Complete set of 176 probes; (b) probes targeting sites relatively free of protein contact; (c) all 176 probes when ΔG°3 estimates were mixed to maximize r2. The solid diamond, circle, and square symbols denote probes Eco 1014, Eco 1184, and Eco 1274, respectively.
