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. 2016 Aug 24;8(4):5874–5884. doi: 10.18632/oncotarget.11580

Figure 4. Ponatinib enhances the cytotoxic effect of Dox on NB cell lines.

Figure 4

A. NGP, SK-N-AS, and LA-N-6 cells were seeded in 96-well plates and were incubated with the indicated concentrations of Dox plus DMSO or 1 μM of ponatinib for 48 hrs. Cell viability was then measured by CCK-8 assay. B. NGP, SK-N-AS, and LA-N-6 cells were treated with either Dox (1 μM) alone, ponatinib (1 μM) alone, or their combination for 0-16 hrs, subjected to SDS-PAGE, and immunoblotted with PARP and Caspase-3 antibodies. β-Actin antibodies were used as a loading control for whole cell extracts in all samples.