TABLE 1.
Purification of propionicin from P. freudenreichii LMGT 2946
| Purification step | Volume (ml) | A280 | Antimicrobial activity (AU/ml) | Specific activity (AU/ml/A280) | Severalfold increase in specific activity |
|---|---|---|---|---|---|
| Culture supernatant | 2,000 | 20.1 | 1,256 | 62.5 | 1 |
| Ammonium sulphate precipitate | 400 | 12.0 | 2,560 | 2.1 × 102 | 3.4 |
| Anion-exchange chromatography | 100 | 1.0 | 2,560 | 2.5 × 103 | 40.0 |
| Reverse-phase chromatography (RESOURCE-I) | 5 | 1.2 | 10,000 | 8.3 × 103 | 132.8 |
| Reverse-phase chromatography (RESOURCE-II) | 2 | 0.09 | 12,800 | 1.4 × 105 | 2,240.0 |
| Reverse-phase chromatography (Sephasil-C8) | 1 | 0.016 | 12,500 | 7.8 × 105 | 12,480.0 |