TABLE 3.
Antimicrobial activity of propionicin F and distribution of the pcfa genea
| Indicator species | Straina | Presence of pcfAb | Sensitivity to propionicin F (MIC [nM]) |
|---|---|---|---|
| P. acidipropionici | ATCC 4965 | − | >320 |
| P. acidipropionici | ATCC 4875 | − | >320 |
| P. freudenreichii ssp. freudenreichii | ATCC 6207T | − | 0.7 |
| P. freudenreichii ssp. shermanii | ATCC 9614T (DSM 4902) | − | 5.0 |
| P. freudenreichii | ATCC 9616 | − | 20 |
| P. freudenreichii | IFO12426 | − | 2.5 |
| P. freudenreichii | LMGT 2842 | − | 5.0 |
| P. freudenreichii | LMGT 3001 | − | 5.0 |
| P. freudenreichii | LMGT 2946 | + | 80, >640c |
| P. freudenreichii | LMGT 2956 | + | 80, >640c |
| P. freudenreichii | ISU-P24 | − | 0.7 |
| P. freudenreichii | ISU-P59 | − | 2.5 |
| P. freudenreichii | ISU-P83 | − | 5.0 |
| P. freudenreichii | ISU-P98 | − | 1.3 |
| P. freudenreichii | ISU-P104 | − | 0.2 |
| P. jensenii | ATCC 4868T | − | >320 |
| P. jensenii | ATCC 14072 | − | >320 |
| P. thoenii | ATCC 4872 | − | >320 |
| P. thoenii | ATCC 4874 | − | >320 |
a
Abbreviations: ATCC, American Type Culture Collection (Rockville, Md.); DSM, Deutsche Sammlung von Mikroorganismen und Zellkulturen; NCDO, National Collection of Food Bacteria (Reading, United Kingdom); ISU, Iowa State University, Ames, Iowa, United States; IFO, the Institute for Fermentation (Osaka, Japan); LMGT, our strain collection; superscript T, type strain.
b
+, amplification of the expected 350-bp pcfA gene fragment; −, negative PCR amplification result.
c
MIC values correspond to strains LMGT 2946 and LMGT 2956 precultivated in SLB and MRS, respectively.