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. 2016 Dec 15;8(4):5943–5953. doi: 10.18632/oncotarget.13966

Figure 2. miR-411 and miR-540-3p increase the C2C12 myotube diameters.

Figure 2

(A) Myostatin knockout mice show a significant increase in the skeletal muscle mass. (B) Confluent C2C12 cells were differentiated into myotubes and transfected with 25 nM of miRNA mimics. Myotubes were transfected with miR-411, miR-434-5p, and miR-540-3p mimics and immunostained with an anti-myosin heavy chain antibody and DAPI. (C and D) Myotube diameter was measured as described in the materials and methods section and was shown as the percent of the control. Transfection of miR-411 and miR-540-3p mimics, but not other miRNAs, significantly increased the myotube diameter. Bar, 250 μm. Error bars indicate the SD of 3 independent experiments. *P < 0.05.