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. 2016 Dec 15;8(4):5943–5953. doi: 10.18632/oncotarget.13966

Figure 4. Myostatin deficiency increases transcription at the Dlk1-Dio3 locus.

Figure 4

(A) Quantitative RT-PCR analysis shows a significant increase in the expression of the primary transcript of miR-127 in myostatin-deficient skeletal muscle at 13 weeks of age. (B) Myostatin deficiency increases the expression levels of the maternally expressed Gtl2 and Rian, as well as the paternally expressed Dlk1. The results were normalized to Rpl26 expression. Data are the mean ± SD (n = 5). *P < 0.05. (C) Strand-specific RT-PCR shows increased expression of Rtl1 and Rtl1as in myostatin-deficient skeletal muscle. (D) Quantitative analyses of Rtl1 and Rtl1as expression using Image J software. The wild-type mice are the same age as the myostatin knockout mice. Data are the mean ± SD (n = 3). **P < 0.01, ***P < 0.001.