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. 2016 Nov 15;8(4):5976–5991. doi: 10.18632/oncotarget.13355

Figure 3. STC2 strengthens HNSCC cell migration, invasion, and tumor metastasis.

Figure 3

A, B. Detection of cell migration by using a high throughput screening multi-well insert 24-well two-chamber plates. Five images were taken randomly for each experiment, and each experiment was repeated three times. Magnification, ×200 (A). Quantitative analysis of the number of migrated cells. (**P < 0.01 and ***P < 0.001.) Error bars = 95%CIs (B). C, D. Representative images of the invasion assay. Magnification, × 200. (**P < 0.01and***P < 0.001). Error bars = 95%CIs. E, F. Detection of migration by scratching assay. Cells were incubated in 6-well plate over-night to yield monolayer confluence. Quantitative analysis of migration speed using migration index (P < 0.05). Error bars = 95% CIs.