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. 2016 Nov 15;8(4):5976–5991. doi: 10.18632/oncotarget.13355

Figure 5. STC2 modulates HNSCC metastasis through the PI3K/AKT/Snail signaling.

Figure 5

A. Detection of molecules associated with PI3K/AKT signaling pathway and EMT program in cell lines by Western blotting. β-actin was used as a loading control. B. Detection of pAKT, Snail, Vimentin and E-cadherinin cell lines after treated with1 μM of MK-2206 for 72h. C-F. AMC-HN-8 and Cal-27-STC2 cells were treated with DMSO or MK-2206 and then used for migration assay. (**P < 0.01 and ***P < 0.001). G. Interruption of Snail expression in AMC-HN-8 scr and Cal-27-STC2 cells altered the expressions of Snail, Vimentin and E-cadherin. H, I. Detection of cell migration by using a high throughput screening multi-well insert 24-well two-chamber plates (H). Quantitative analysis of migrated cells (*P< 0.05and **P < 0.01) (I).