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. 2016 Dec 20;8(4):6102–6113. doi: 10.18632/oncotarget.14043

Figure 5. Idelalisib synergizes with 5-FU or regorafenib to induce apoptosis via PUMA in colon cancer cells.

Figure 5

(A) HCT116 cells were treated with 5 μmol/L idelalisib, 20 mg/L 5-FU, or their combination for 24 hours. PUMA and cleaved-caspase 3 expression were analyzed by Western blotting. (B) Parental and PUMA-KD HCT116 cells were treated 5 μmol/L idelalisib , 20 mg/L 5-FU, or their combination for 24 hours. Apoptosis was analyzed by a nuclear fragmentation assay. (C) HCT116 cells were treated with 5 μmol/L idelalisib, 20 mg/L regorafenib, or their combination for 24 hours. PUMA and cleaved-caspase 3 expression were analyzed by Western blotting. (D) Parental and PUMA-KD HCT116 cells were treated 5 μmol/L idelalisib , 20 mg/L regorafenib, or their combination for 24 hours. Apoptosis was analyzed by a nuclear fragmentation assay. Results in (B) and (D) were expressed as means ± SD of 3 independent experiments. ***P < 0.001;**P < 0.01; *P < 0.05.