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. 2016 Dec 20;8(4):6142–6154. doi: 10.18632/oncotarget.14046

Figure 6. Bone marrow failure model mediated by infusion of wild-type or miR34a−/− lymph node cells.

Figure 6

Lymph node (LN) cells from wild-type or miR34a−/− donors were used as effectors to induce BM failure in sublethally irradiated (5 Gy) CB6F1 recipient mice. F1 mice were treated with total body irradiation (TBI) only (n = 8), TBI with wild-type (WT) LN cells (n = 12), or TBI with miR34a−/− LN cells (n = 12). Total BM cells were calculated assuming bilateral tibias, ilia, and unilateral femur contain 25% of total marrow cells. (A) Representative hematoxylin and eosin femur section (original magnification 200 ×). The three groups were compared in total BM cells, LSK cell percentage, and total LSK cells shown as medians with ranges (B) and representative scatterplots (C). T cell expansions in the BM are shown as representative histograms (D) and means with SEMs (E). Infused wild-type LN cells expanded more vigorously than miR34a−/− LN cells. Data are representative of three experiments. *P < 0.05; ns, no significance.