Figure 3. CITED2 silencing attenuates expression of VEGFA isoforms in MDA-MB-231 tumors.
(A and B) Left: mRNA expression of VEGFA isoforms in the orthotopic tumors (A) and cells grown in vitro (B) as determined by qRT-PCR (*P < 0.05, **P < 0.01). Right: Top, Western blot analysis of VEGFA isoforms performed on equal amounts of protein obtained from total cell lysates of orthotopic tumor. GAPDH served as the loading control. Bottom, Western blot analysis of VEGFA isoforms performed on equal amounts of protein obtained from serum-free conditioned media of cells grown in vitro.
