Skip to main content
. 2016 Dec 21;8(4):6319–6329. doi: 10.18632/oncotarget.14062

Figure 4. HDACs 1 and 2 cooperate in regulating BRCA1, CHK1, and RAD51 expression in AML cells.

Figure 4

(A and C) THP-1 cells were infected with HDAC1 (THP-1/HDAC1), HDAC2 (THP-1/HDAC2), HDAC3 (THP-1/HDAC3), or NTC control (THP-1/NTC) shRNA lentivirus overnight, then washed and incubated for 48 h prior to adding puromycin to the culture medium. Whole cell lysates were subjected to Western blotting and probed with the indicated antibodies. The fold changes for the densitometry measurements, normalized to β-actin and then compared to no drug treatment control, are indicated. These Western blots were previously published [15]. (B) Total RNAs were isolated from the transfected cells and gene transcript levels were determined by Real-time RT-PCR. Transcript levels were normalized to GAPDH and relative expression levels were calculated using the comparative Ct method. (D and E) THP-1/HDAC1 and THP-1/HDAC2 cells were treated with RGFP966 for 48 h. Whole cell lysates were subjected to Western blotting and probed with the indicated antibodies.