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. 2016 Dec 21;8(4):6319–6329. doi: 10.18632/oncotarget.14062

Figure 5. FK228 decreases expression of BRCA1, CHK1, and RAD51 by inhibiting HDAC1 and HDAC2 in AML cells.

Figure 5

(A and B) THP-1 and OCI-AML3 cells were treated with FK228 for 48 h and whole cell lysates were subjected to Western blotting and probed with the indicated antibodies. The fold changes for the densitometry measurements, normalized to β-actin and then compared to no drug treatment control, are indicated. (C) THP-1 and OCI-AML3 cells were treated with FK228 for 48 h and then subjected to Annexin V-FITC/PI staining and flow cytometry analyses. **indicates p < 0.01 and ***indicates p < 0.001. (D and E) THP-1 and OCI-AML3 cells were treated with FK228 for 48 h. Total RNAs were isolated from treated cells and gene transcript levels were determined by Real-time RT-PCR. Transcript levels were normalized to GAPDH and relative expression levels were calculated using the comparative Ct method. **indicates p < 0.01 and ***indicates p < 0.001.