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. 2016 Dec 22;8(4):6353–6363. doi: 10.18632/oncotarget.14087

Figure 5. FLNC knock-down downregulates Rac1 and Cdc42 activation.

Figure 5

Rho GTPase activities were evaluated in total lysates by activated GTPase pull-down. FLNC knockdown was associated with suppression of GTP-Rac1 and GTP-Cdc42, but not GTP-RhoA in both TE-1 and TE-8 cells. Densities of the individual bands were quantified using Image J software. The densities of GTP-forms bands were normalized to the densities of bands of each total Rho family protein. Relative densities (RD)were obtained by comparing to the density of each GTP-form band of Ssh infected cells.