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. 2016 Dec 22;8(4):6376–6398. doi: 10.18632/oncotarget.14093

Figure 3. Iron responsive protein/Iron responsive element (IRP/IRE) components are deregulated and show activation of the IRP/IRE binding in tumor-initiating cells (TICs).

Figure 3

Expression of the ACO1 gene at the mRNA level in breast non-malignant cell line MCF10A, in TICs derived from breast cancer cell lines MCF-7, BT-474, T-47D and ZR-75-30 as well as from prostate cancer cell lines DU-145 and LNCaP has been determined (A) together with protein levels in the MCF-7 cell line (CTRL) and MCF-7 derived spheres (SPH) (B). Similarly, the expression of the IREB2 gene at the mRNA (C) level as well as protein level (D) in TICs is documented. Experiments were performed at least in triplicate, standard error is SEM, p-values lower than 0.05 are denoted with a star and were calculated by the GenEx software using the unpaired t-test and plotted with GraphPad prism software. Number sign denotes statistical significance involving Dun-Bonferroni correction. Panel (E) illustrates the IRP/IRE activity measured by the fluorescent EMSA. The protein expression was quantified by the image J software from 2 to 5 independent samples, standard error is SEM, p-values lower than 0.05 are denoted with a star and were calculated and plotted in GraphPad prism, using the unpaired t-test.