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. 2016 Dec 23;8(4):6399–6405. doi: 10.18632/oncotarget.14113

Figure 1. Step II: PNA clamping experimental design.

Figure 1

Template amplification of CALR wild-type (WT), type-1 (DEL) and type-2 (INS) mutations (A) in absence of PNA probe (B) and with PNA probe. Perfect PNA/DNA hybridization occurs when CALR template sequence is wild-type (WT) or type-1 mutation (DEL, indicated by [¨¨]), thus leading to a significant reduction of PCR amplification. By contrast, in presence of type-2 mutation (INS, indicated by +++++), PNA/DNA duplex is highly destabilized, on behalf of primer Competitor Rev, allowing strong template amplification.