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. 2016 Dec 23;8(4):6461–6474. doi: 10.18632/oncotarget.14120

Figure 4. EBV inoculation elicits ATR/Chk1-mediated pathway activation but not ATM/Chk2 in peripheral B-cells (PBCs).

Figure 4

Peripheral B-cells were inoculated with EBV-B95.8 (MOI 8). Non-inoculated peripheral B-cells at 24 hours post isolation served as negative control (Uninfected), Lymphoblastoid cell lines (LCLs) derived from TMCs or PBMCs treated with Etoposide for 1 hours served as positive control. Detection of MRE11 served as loading control. (A) Cells were harvested at the indicated time points and expression of total ATR or Chk1 and pATR S428 or pChk1 S345 was analyzed by western blotting. (B) Cells were harvested at the indicated time points, and expression of total ATM or Chk2 and pATM S1981 or pChk2 T68 was analyzed by western blotting. The results shown are representative of 3 independent experiments. (C) ATR, ATM, Chk1 and Chk2 phosphorylation and total protein amount were quantified by densitometric analysis. Data are represented as ratio of phosphorylated-to-total. Quantification was performed using the software imageJ 1.49t. * = Not detectable.